Table 2.
Primers for real-time PCR in this study
| Gene1 | Sequence (5ʹ–3ʹ) | Size(bp) | Accession number |
|---|---|---|---|
| ZO-1 | Forward: AGCCCGAGGCGTGTTT Reverse: GGTGGGAGGATGCTGTTG |
147 | XM_013993251 |
| Occludin | Forward: GCACCCAGCAACGACAT Reverse: CATAGACAGAATCCGAATCAC |
144 | XM_005672525 |
| Mucin-2 | Forward: CTGCTCCGGGTCCTGTGGGA Reverse: CCCGCTGGCTGGTGCGATAC |
100 | XM_007465997.1 |
| pBD2 | Forward: CCAGAGGTCCGACCACTACA Reverse: GGTCCCTTCAATCCTGTTGAA |
88 | AY506573.1 |
| PG1-5 | Forward: GTAGGTTCTGCGTCTGTGTCG Reverse: CAAATCCTTCACCGTCTACCA |
166 | XM_005669497.2 |
| NOD1 | Forward: ACCGATCCAGTGAGCAGATA Reverse: AAGTCCACCAGCTCCATGAT |
140 | NM_001114277.1 |
| NOD2 | Forward: CCTTTTGAAGATGCTGCCTG Reverse: GATTCTCTGCCCCATCGTAG |
100 | NM_001105295.1 |
| TLR2 | Forward: TCACTTGTCTAACTTATCATCCTCTTG Reverse: TCAGCGAAGGTGTCATTATTGC |
162 | NM_213761.1 |
| TLR4 | Forward: GCCATCGCTGCTAACATCATC Reverse: CTCATACTCAAAGATACACCATCGG |
108 | NM_001113039.1 |
| β-actin | Forward: CTGCGGCATCCACGAAACT Reverse: AGGGCCGTGATCTCCTTCTG |
380 | XM_003124280 |
| GAPDH | Forward: ACTCACTCTTCCACTTTTGATGCT Reverse: TGTTGCTGTAGCCAAATTCA |
100 | NM_001206359 |
1ZO-1 = zonula occludens-1; pBD2 = porcine β defensin 2; PG1-5 = protegrin 1-5; NOD = nucleotide binding oligomerization domain; TLR = toll-like receptor; GAPDH = glyceraldehyde-3-phosphate dehydrogenase.