Table 4.
Qualitative profile of porcine milk oligosaccharides (OS) identified in all porcine milk samples organized by molecular mass (least to greatest)
No. | Compounda | Massb | RTc | No. | Compound | Mass | RT | No. | Compound | Mass | RT |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2_0_1_0_0 | 488.1723 | 14.84 | 21 | 3_1_0_0_0 | 707.2477 | 15.82 | 41 | 3_2_0_0_0 | 910.3267 | 22.66 |
2 | 3_0_0_0_0 | 504.1685 | 12.74 | 22 | 3_1_0_0_0 | 707.2492 | 17.86 | 42 | 4_0_0_1_0 | 957.3281 | 26.59 |
3 | 3_0_0_0_0 | 504.1683 | 13.38 | 23 | 3_1_0_0_0 | 707.2478 | 20.44 | 43 | 6_0_0_0_0 | 990.3279 | 16.59 |
4 | 3_0_0_0_0 | 504.1684 | 13.97 | 24 | 3_1_0_0_0 | 707.2477 | 22.63 | 44 | 3_1_0_1_0 | 998.3431 | 25.58 |
5 | 3_0_0_0_0 | 504.1685 | 15.02 | 25 | 3_1_0_0_0 | 707.2481 | 28.36 | 45 | 3_1_0_1_0 | 998.3446 | 29.05 |
6 | 3_0_0_0_0 | 504.1683 | 16.22 | 26 | 2_2_0_0_0 | 748.2741 | 17.54 | 46 | 4_1_1_0_0 | 1015.358 | 15.87 |
7 | 2_1_0_0_0 | 545.1948 | 13.44 | 27 | 2_2_0_0_0 | 748.2744 | 15.96 | 47 | 4_1_1_0_0 | 1015.359 | 26.8 |
8 | 2_1_0_0_0 | 545.1949 | 15.77 | 28 | 2_2_0_0_0 | 748.2747 | 19.45 | 48 | 4_1_1_0_0 | 1015.355 | 17.33 |
9 | 2_1_0_0_0 | 545.1948 | 19.14 | 29 | 3_0_0_1_0 | 795.2644 | 26.07 | 49 | 4_2_0_0_0 | 1072.381 | 22.64 |
10 | 1_2_0_0_0 | 586.2208 | 13.42 | 30 | 3_0_0_1_0 | 795.2648 | 27.28 | 50 | 4_2_0_0_0 | 1072.378 | 25.03 |
11 | 1_2_0_0_0 | 568.2211 | 15.08 | 31 | 3_0_0_1_0 | 795.2643 | 24.91 | 51 | 4_2_0_0_0 | 1072.379 | 30.62 |
12 | 2_0_0_1_0 | 633.2116 | 19.03 | 32 | 5_0_0_0_0 | 828.2748 | 15.4 | 52 | 3_3_0_0_0 | 1113.406 | 20.77 |
13 | 2_0_0_1_0 | 633.2116 | 23.92 | 33 | 2_1_2_0_0 | 837.3014 | 24.38 | 53 | 3_3_0_0_0 | 1113.404 | 23.44 |
14 | 4_0_0_0_0 | 666.2209 | 15.69 | 34 | 4_1_0_0_0 | 869.3016 | 20.43 | 54 | 4_1_0_1_0 | 1160.397 | 28.27 |
15 | 4_0_0_0_0 | 666.2229 | 20.55 | 35 | 4_1_0_0_0 | 869.3015 | 21.6 | 55 | 3_2_0_1_0 | 1201.42 | 27.13 |
16 | 4_0_0_0_0 | 666.2217 | 21.47 | 36 | 4_1_0_0_0 | 869.3007 | 28.28 | 56 | 4_2_1_0_0 | 1218.437 | 18.95 |
17 | 4_0_0_0_0 | 666.2215 | 13.55 | 37 | 2_2_1_0_0 | 894.3348 | 12.08 | 57 | 4_2_1_0_0 | 1218.436 | 20.56 |
18 | 1_1_0_1_0 | 674.2345 | 17.2 | 38 | 3_2_0_0_0 | 910.3268 | 18.43 | 58 | 4_3_0_0_0 | 1275.456 | 22.28 |
19 | 1_1_0_1_0 | 674.2374 | 18.96 | 39 | 3_2_0_0_0 | 910.3271 | 19.37 | 59 | 4_2_0_1_0 | 1363.481 | 27.09 |
20 | 1_1_0_1_0 | 647.2378 | 23.48 | 40 | 3_2_0_0_0 | 910.3271 | 20.19 | 60 | 4_2_0_1_0 | 1363.478 | 30.63 |
61 | 4_3_0_1_0 | 1566.55 | 26.22 |
aThe compound name, or composition of the OS, is shown as a set of 5 monomers. The following order is as stated with their abbreviations: hex = glucose or galactose; HexNAc = N-acetylhexososamine; Fuc = Fucose; Neu5Ac = N-acetylneuramic acid; and Neu5Gc = N-glycolylneuramic acid (e.g., 3_1_0_0_0 is equal to 3 Hex, 1 HexNAc).
bMass is the molecular mass measured by the mass spectrometer.
cRetention time (RT) is the specific elution time from the LC analytical column for each compound.