Extended Data Figure 8. Loss of Aspm disrupts centriole duplication in ferret embryonic fibroblasts.
Mitotic Aspm KO FEF, identified by staining for pH3 and co-stained for the centriolar marker Centrin, display a significant loss of centrioles. The percentage of cells with an abnormal number (less than 4) of centrioles is increased 8-fold in Aspm−/− FEF compared to Aspm+/+ FEF (n = 100 cells/genotype/independent experiments; P = 0.003). The experiments were repeated 3 times independently with similar results. Statistical analysis was performed by two-tailed t-test and the graph shows mean ± s.e.m.