Skip to main content
. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Nature. 2018 Apr 11;556(7701):370–375. doi: 10.1038/s41586-018-0035-0

Figure 2. Aspm KO ferrets show displaced NPC.

Figure 2

a–f, Nuclear staining of Aspm−/− ferrets shows a premature OSVZ-like zone (a–c, arrowheads) containing NPC that express Pax6, Sox2, and Ki67 (d–f). g–k, Displaced NPC include Sox2+/pVim+ ORG (g, arrowheads) with a basal process (g, arrows; h, i, k), and Tbr2+ IP. Abventricular pVim+ NPC are increased 3-fold in Aspm−/− ferrets (j) (*, P = 0.006 by one-tailed t-test; mean ± s.e.m; n = 3 +/− and n = 4 −/− animals). l–o, Displaced NPC express Vim, Eomes, or Ptprz1 (l, m), have Arl13b+ cilia (o), and are either Sox2+/Neurog2+/Hopx+ (filled arrowheads) or Sox2+/Neurog2+/Hopx- (open arrowheads) (n). p, q, Aspm−/− mice lack displaced NPC. See Methods for statistics and reproducibility. Scale bars: a–c, 500 μm; d–f, 50 μm; g–i, n, o, 10 μm; k–m, p, q, 100 μm.