Table 1.
Antibodies Used in the Study
| Antibodies | Epitopes | Dilution | References and Sources |
|---|---|---|---|
| CIP2A (poly) | CIP2A | 1/1,000 | ABclonal (Boston, MA, USA) |
| CIP2A (poly) | CIP2A | 1/200–1,000 | Cell Signaling (Danvers, MA, USA) |
| pT668 (poly) | APP (Thr668) | 1/1,000 | Cell Signaling (Danvers, MA, USA) |
| DM1A (mAb) | α-tubulin | 1/500 | Cell Signaling (Danvers, MA, USA) |
| PSD95 (poly) | PSD95 | 1/1,000 | Cell Signaling (Danvers, MA, USA) |
| GAPDH (poly) | GAPDH | 1/1,000 | Cell Signaling (Danvers, MA, USA) |
| PP2Aa (poly) | P2A A subunit | 1/1,000 | Cell Signaling (Danvers, MA, USA) |
| APP (mAb) | APP | 1/1,000 | Droteintech (Chicago, IL, USA) |
| BACE1 (poly) | BACE1 | 1/500 | Droteintech (Chicago, IL, USA) |
| PS1 (poly) | PS1 | 1/500 | Proteintech (Chicago, IL, USA) |
| sAPPα (mAb) | sAPPα/fragments | 1/500 | Immuno-Biological Laboratories (Gunma, Japan) |
| sAPPβ (poly) | Sappβ/fragments | 1/500 | Immuno-Biological Laboratories (Gunma, Japan) |
| PP2Ac (mAb) | PP2AC | 1/1,000 | Millipore (Billerica, MA, USA) |
| GluA1 (poly) | GluA1 protein | 1/500 | Millipore (Billerica, MA, USA) |
| GluA2 (poly) | GluA2 (aa 175–430) | 1/500 | Millipore (Billerica, MA, USA) |
| N-GluA1 (mAb) | GluA1 N terminus | 1/100 | Millipore (Billerica, MA, USA) |
| Synapsin 1 (poly) | Synapsin 1 | 1/1,000 | Millipore (Billerica, MA, USA) |
| Tau-5 (mAb) | Tau-5 (aa 210–241) | 1/1,000 | Millipore (Billerica, MA, USA) |
| Tau-5 (mAb) | - | 1/1,000 | Thermo Fisher (Waltham, MA, USA) |
| Synaptophysin (poly) | Synaptophysin (aa 250–350) | 1/1,000 | Abcam (Cambridge, UK) |
| β-Actin (mAb) | β-actin | 1/1,000 | Abcam (Cambridge, UK) |
| pS396 (mAb) | Tau-5(aa 352–441) | 1/1,000 | Anaspec (Fremont, CA, USA) |
| pS396 (poly) | Tau (Ser 396) | 1/200–1,000 | Signalway Antibody (Pearland, TX, USA) |
| pS404 (poly) | Tau (Ser 404) | 1/200–1,000 | Signalway Antibody (Pearland, TX, USA) |
aa, amino acids; mAb, mouse monoclonal antibody; p, phosphorylated; poly, rabbit polyclonal antibody.