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. Author manuscript; available in PMC: 2018 Nov 16.
Published in final edited form as: Cell. 2017 Nov 16;171(5):1206–1220.e22. doi: 10.1016/j.cell.2017.10.019

Figure 1. Single-cell RNA-seq Protocol for the Drosophila Pupal Brain.

Figure 1.

(A) Schematic of fly olfactory system organization. Olfactory receptor neurons (ORNs) expressing the same odorant receptor (same color) target their axons to the same glomerulus in the antennal lobe. Projection neuron (PN) dendrites also target single glomeruli, and their axons project to the mushroom body (MB) and lateral horn (LH).

(B) Schematic of single-cell RNA-seq protocol.

(C) Representative confocal images of Drosophila central brains labeled by UAS-mCD8GFP crossed with PN driver GH146-GAL4 (24h APF) or astrocyte driver alrm-GAL4 (72h APF). N-cadherin (Ncad, red) staining labels neuropil. Scale, 50 μm.

(D) Heat map showing expression levels of genes that are specific for neurons or astrocytes. Each column is an individual cell. 67 alrm-GAL4+ and 946 GH146-GAL4+ cells are shown, with driver indicated by the color above. Cell type-specific genes are enriched in astrocytes (top 9) and PNs (bottom 5). Expression levels are indicated by the color bar (CPM, counts per million). Cells and genes were ordered using hierarchical clustering.

(E) Visualization of astrocyte and PN populations using t-distributed Stochastic Neighbor Embedding (tSNE). Each dot is a cell.See also Figure S1.