REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Ratanti-DNcad	Developmental Studies Hybridoma Bank	DN-Ex#8
Chicken anti-GFP	Aves Labs	GFP-1020
Rabbit anti-DsRed	Clontech	632496
Ratanti-C15	(Campbell, 2005]	N/A
Guinea pig anti-knot	Jinushi-Nakao et al., 2007	N/A
Bacterial and Virus Strains
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Critical Commercial Assays
Deposited Data
Sequencing reads	This paper	GSE100058
Preprocessed sequence data	This paper	GSE100058
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
D. melanogaster: Mzl9-GAL4	Bloomington Drosophila Stock Center	BDSC: 34497
D. melanogaster: Alrm-GAL4	Bloomington Drosophila Stock Center	BDSC: 67032
D. melanogaster:C15-RNAI #1	Bloomington Drosophila Stock Center	BDSC: 27649
D. melanogaster:C15-RNAI #2	Bloomington Drosophila Stock Center	BDSC: 35018
D. melanogaster:GH146-GAL4	(Stacker etal., 1997)	BDSC: 30026
D. melanogaster: UAS-STOP-mCDSGFP	Potter etal., 2010	BDSC: 30125
D. melanogaster: UAS-STOP-mCDSGFP	(Hong etal., 2012)	BDSC: 41573
D. melanogaster: 91G04-GAL4	(Jenett etal., 2012)	BDSC: 40588
D. melanogaster: trol-GAL4	Kyoto Stock Center	DGRC: 113584
D. melanogaster: GH146-Flp	(Hong etal., 2009)	N/A
D. melanogaster: unpg-lacZ	(CuiandDoe, 1995)	N/A
D. melanogaster: UAS-C15	(Campbell, 2005)	N/A
D. melanogaster: nos-Cas9	Diao etal., 2015	N/A
D. melanogaster: CG31676-GAL4	This paper	N/A
Oligonucleotides
Actin5C (qPCR forward primer): 5’-CTCGCCACTTGCGTTTACAGT-3’	This paper	N/A
Actin5C (qPCR reverse primer): 5’-TCCATATCGTCCCAGTTGGTC−−3’	This paper	N/A
C15 (qPCR forward primer): 5’- AGCGCTTCCACAAGCAAAAG-3’	This paper	N/A
C15 (qPCR reverse primer): 5’- CCGTCTGTCGTCTCCACTTG-3’	This paper	N/A
Recombinant DNA
Plasmid: pT-GEM(l)	Addgene	62893
Plasmid: PU6-BbsI-ChiRNA	Addgene	45946
Plasmid: TOPO-CG31676-T2A-GAL4	This paper	N/A
Software and Algorithms
Custom analysis software	This paper	https://github.com/felixhorns/FlyPN
Iterative Clustering for Identifying Markers	This paper	https://github.com/felixhorns/FlyPN
Other