Fig 1.

A) Study participant composition in United States and Morocco. One seropositive individual was tested via POC test twice but is included in this number only once. 19 pregnant women were tested a total of three times each as part of a pilot gestational screening program. All were seronegative. Volunteers learned of the study through word of mouth—sometimes when friends or family members knew of an affected child and asked to participate—or were formally recruited in an obstetrical practice. No one complained or mentioned medical problems of any type other than as related to toxoplasmosis among those who were seropositive from the NCCCTS or pregnancy, although there was no health questionnaire or physical examination as part of this study. B) Performing the Toxoplasma ICT IgG–IgM Test. The site of the fingerstick is cleansed with an alcohol wipe and pricked with a lancet. Blood is collected via capillary tube and applied to the test kit. Four drops of eluent are then applied. Test can be interpreted in 20–30 minutes. The black arrow adjacent to the “T” indicates the presence of antibodies against T. gondii. NCCCTS, National Collaborative Chicago-Based Congenital Toxoplasmosis Study; POC, point of care; UofC, University of Chicago.