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. 2018 Aug 16;14(8):e1007545. doi: 10.1371/journal.pgen.1007545

Fig 3. LacCer and GlcCer synthase activity and sphingolipid analysis in DKO mice.

Fig 3

(A) LacCer synthase activity was measured in brain homogenates from wild-type (wt) and B4galt5 and 6 double KO (DKO) mice (n = 3 per genotype) at 3 weeks of age. (B) GlcCer synthase activity was measured in brain homogenates from the same mice in A (n = 3 per genotype). The levels of GlcCer (C) and GalCer (D) in brain homogenates of the same mice as in (A) and (B) were measured (n = 3 per genotype). (E) The levels of total SM, Cer, Sph, and DHS in brain homogenates from wt and DKO mice (wt, n = 4; DKO, n = 2) at 3 weeks of age. Note that small squares on the bar graph indicate the individual values of each sample. (F) GSLs extracted from brain homogenates from wt and DKO mice at 3 weeks of age were separated by high performance thin layer chromatography (n = 3 per genotype). M1 and M2, standard GSLs are indicated. *, p<0.05; **, p<0.01.