Table 3. Keratinase activity in the presence of reducing agents.
| Enzyme | Reducing agent | pH | Specific Activity (103 KU/g of protein) |
Fold change in activity |
|---|---|---|---|---|
| Cibenza DP100 | No reducing agent | 8 | 16 ± 1 | - |
| 1% sulfite | 8 | 18 ± 1 | 1.1 | |
| 2% sulfite | 8 | 18 ± 1 | 1.1 | |
| 1% thioglycolate | 8 | 22 ± 2 | 1.4 | |
| 2% thioglycolate | 8 | 23 ± 2 | 1.4 | |
| 5% thioglycolate | 8 | 22 ± 2 | 1.4 | |
| Ronozyme ProAct | No reducing agent | 10 | 262 ± 5 | - |
| 1% sulfite | 10 | 338 ± 2 | 1.3 | |
| 2% sulfite | 10 | 235 ± 3 | 0.9 | |
| 1% thioglycolate | 10 | 470 ± 14 | 1.8 | |
| 2% thioglycolate | 10 | 684 ± 60 | 2.6 | |
| 5% thioglycolate | 10 | 391 ± 18 | 1.5 |
Data are represented as mean values ± standard deviation (n = 3).