Fig 3. SLC25A25 is a Ca²⁺-regulated Mg-ATP transporter.

(A) Purified SLC25A25 (5 μg) was separated by SDS-PAGE and visualized by Coomassie staining. (B) Thermostability profile (top) and its corresponding first derivative (bottom) of SLC25A25. Assays were performed in triplicate with the addition CaCl₂ (orange line) or without (blue line). (C) Uptake assays of [¹⁴C]-labeled Mg-ATP into proteoliposomes with the addition CaCl₂ (orange line) and without (blue line). The error bars represent the standard deviation of 4 replicates, and the uptake curves were fitted with a one-phase association curve (r-squared > 0.99). For numerical values, see S1 Data. AU, arbitrary unit; SLC25A25, solute carrier 25 A 25.