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. 2018 Aug 6;14(8):e1007206. doi: 10.1371/journal.ppat.1007206

Fig 8. Exonic structure of circLMP2_E8_E2.

Fig 8

A) Coverage and forward splicing enrichment of LMP2 exons 2 through 8 in RNase R-seq in reactivated Akata cells suggests exons 2 through 8 inclusion in circLMP2_E8_E2. Forward splicing (over arches), coverage (exon color intensity), and backsplicing (under arches) derived from polyA-seq and RNase R-seq in reactivated Akata cells and JY cells. B) RT-PCR using forward primers in exons 4 through 7 support a sequentially forward-spliced configuration of circLMP2_E8_E2. All PCR reactions were repeated with similar results. C) Nuclear/cytoplasmic distribution of circLMP2_E8_E2 using RT-qPCR. Cytoplasmically localized ACTB and nuclear localized KCNQ1OT1 are shown for comparison. Fraction cytoplasmic is relative to ACTB (cytoplasmic) and KCNQ1OT1 (nuclear). Error bars represent standard deviations and were derived from triplicate qPCR reactions. These experiments were repeated once with similar results. Method for calculating fraction cytoplasmic is outlined in the methods section.