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. 2018 Aug 6;12(8):e0006673. doi: 10.1371/journal.pntd.0006673

Fig 2. Assay parameters determination.

Fig 2

A. D-luciferin concentration in relation to RLU. Serial concentration of D-luciferin relative to the wild-type strain to determine the optimal concentration of D-luciferin to use in assays. The reaction was carried out for 30 min. The line shows the RLU data-points at 10 mg/ml of D-luciferin. (RLU: relative light units). B. Optimization of incubation time. D-luciferin concentration in relation to relative light units (RLU) was measured at different time points. C. Parasite concentration and RLU values. The plot showed bioluminescence read out from the wild type strain and the transfectant strain correlating number of cells to RLU. The values of both the luciferase strain and wild-type strain are shown with respective RLU at 30 min. (P-value of luciferase strain < 0.001: Wild-type p = 0.854). RLU versus parasite concentration helps to determine the ideal maximal end concentration without overgrowth carrying out the reaction for 30 min.