Table 2. Representative set of dual hits against Giardia and Cryptosporidium with ≥ 75% inhibition.
EC50: 50% effective concentration, CC50: 50% cytotoxic concentration, % inh: percentage inhibition.
| Common name | MMV Compound ID | G. lamblia EC50 (μM) | C. parvum EC50 (μM) | HepG2 CC50 (μM) (this study) | HepG2 CC50 (μM) (literature values) |
|---|---|---|---|---|---|
| Iodoquinol | MMV002817 | 2.52 ± 0.45 | 0.48± 0.2 | ~40 | 2.50*^ |
| N/A | MMV010576 | 1.9 ± 0.28 | 2.99 ± 2.1 | >40 | >10* |
| N/A | MMV028694 | 3.88 ± 1.27 | 1.56 ± 0.5 | 15.87 | 8.10* |
| N/A | MMV676501 | 1.40 ± 0.2 | 4.96 ± 1.8 | >40 | 50.10* |
| Clofazimine | MMV687800 | 1.79 ± 0.2 | 3.33 ± 1.2 | 26.43 | >20+ |
| Auranofin | MMV688978 | 3.74 ± 0.46 | 3.34 ± 2.7 | 0.50 | 4.50# |
| Nitazoxanide | MMV688991 | 0.80 ± 0.10 | 3.10 ± 0.4 | 10.73 | 6.35*^ |
* Pathogen_Box_Activity_Biological_Data__ Structure & Smiles (https://www.pathogenbox.org/about-pathogen-box/composition)
N/A–not applicable;
^ HepG2 CC20 (μM)
+ reference [41];
# reference [56]