KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-SH3GL3 | Abcam | Cat#: ab184008 |
| Mouse monoclonal anti-SH3GL3 | Santa Cruz Biotech | Cat#: SC-376592 |
| Rabbit polyclonal anti-TIAM1 | Santa Cruz Biotech | Cat#: SC-872 |
| Mouse monoclonal anti-TIAM1 | Santa Cruz Biotech | Cat#: SC-393315 |
| Mouse monoclonal anti-Rac1 | Cytoskeleton Inc. | Cat#: ARC03 |
| Mouse monoclonal anti-FLAG | Sigma | Cat#: F-3165 |
| Rabbit polyclonal anti-His6 tag | Abcam | Cat#: ab9108 |
| Rabbit Polyclonal anti-Ki-67 | Abcam | Cat#: ab16667 |
| Mouse monoclonal anti-TROMA-1 | DSHB | Cat#: TROMA-1 |
| Rabbit polyclonal anti-dsRed | Takara | Cat#: 632496 |
| Mouse monoclonal anti-TIAM1 | FHCRC Shared Resource | 4E7b |
| Bacterial and Virus Strains | ||
| BL21 (DE3) Escherichia Coli | Thermo Fisher Scientific | Cat#: C601003 |
| Mach1 T1 Escherichia Coli | Thermo Fisher Scientific | Cat#: C8681201 |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Dako serum free block | Agilent | Cat#: X090930-2 |
| Acti-Stain 488 Phalloidin | Cytoskeleton Inc | Cat#: PHDG1-A |
| DAPI | Sigma | Cat#: D9542 |
| Malachite Green Oxalate Salt | Sigma | Cat#: M6880 |
| Prolong Diamond antifade reagent | Molecular Probes | Cat#: P36965 |
| Live Imaging Solution | Thermo Fisher Scientific | Cat#: A14291DJ |
| 0.25% Trypsin EDTA | Thermo Fisher Scientific | Cat#: 25200-056 |
| Opti-MEM Media | Thermo Fisher Scientific | Cat#: 31985062 |
| Leibovitz's L-15 Medium | Thermo Fisher Scientific | Cat#:11415064 |
| Collagen Type 1 | Thermo Fisher Scientific | Cat#: A1048301 |
| Matrigel | Corning | Cat#: 354234 |
| Fetal Bovine Serum | Omega Scientific | FB-01 |
| 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) | NOF America | Cat#: ME-6060 |
| 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) | NOF America | Cat#: MC-6081 |
| 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) | NOF America | Cat#: MS-8181LS |
| 1,2-dioleoyl-sn-glycero-3-phospho-(1'-myo-inositol-4',5'-bisphosphate) (PIP2) | Avanti Polar Lipids | Cat#:850155 |
| Recombinant EndoA1 BAR domain | Laboratory of Jihong Bai | BJP-B01 |
| Recombinant EndoA2 BAR domain | Laboratory of Jihong Bai | BJP-B595 |
| Recombinant EndoA3 BAR domain | Laboratory of Jihong Bai | BJP-B596 |
| Recombinant Full Length EndoA1 | Laboratory of Jihong Bai | BJP-B237 |
| Recombinant Full Length EndoA2 | Laboratory of Jihong Bai | BJP-R41 |
| Recombinant Full Length EndoA3 | Laboratory of Jihong Bai | BJP-R42 |
| Recombinant TIAM1 (His6-PDZ-DH) domain | This paper | BJP-674 |
| Recombinant PAK-PBD | This paper | BJP-A144 |
| Recombinant RAC G15A | This paper | BJP-R85 |
| DMEM | Thermo Fisher Scientific | Cat#: 11965092 |
| Critical Commercial Assays | ||
| Incucyte zoom system | Essen Bioscience | Cat#: 4647 |
| FM 1–43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) | Thermo Fisher Scientific | Cat#: T3163 |
| FM 4–64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) | Thermo Fisher Scientific | Cat#: T13320 |
| Lipofectamine RNAiMAX | Thermo Fisher Scientific | Cat#: 13778030 |
| Lipofectamine 2000 | Thermo Fisher Scientific | Cat#: 11668027 |
| RAC/CDC 42 Inhibitor ML141 Inhibitor | Millipore Sigma | Cat#: 217708 |
| PTEN Inhibitor VO-OHpic | Sigma | Cat#: V8639 |
| PTEN Inhibitor bPV(HOpic) | Sigma | Cat#: SML0884 |
| Dynamin Inhibitors Toolbox | Abcam | Cat#: ab120468 |
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Human colon adenocarcinoma cells: DLD1 | ATCC | Cat#: CCL-221 |
| Human colon adenocarcinoma cells: HCT116 | ATCC | Cat#: CCL-247 |
| Human colon adenocarcinoma cells: SW480 | ATCC | Cat#: CCL-228 |
| MLV-based retroviral packaging cell line: PT67 | ATCC | Cat#: CRL-12284 |
| Human Embryonic Kidney Cells | Sigma | Cat#: 85120602 |
| Experimental Models: Organisms/Strains | ||
| D. rerio: WT AB | Laboratory of Cecilia Moens | ZFIN: ZDB-GENO-960809-7 |
| M. musculus: NU/NU | Charles River | Crl:NU-Foxn1nu |
| Oligonucleotides | ||
| 3' for EndoA2 BAR in A2 BAR-A3SH3 chimera | ATGTTCACAGGCTTTGGCATGAACTCCCGCTTGGG | |
| 5' for EndoA2 BAR in A2 BAR-A3SH3 chimera | ATGCCAAAGCCTGTGAACA | |
| 3' for EndoA3 BAR in A3 BAR-A2 SH3 chimera | GGCTCCCGGGGCCGGGGCTTGAAcTCTCGCTTGGGG | |
| 5' for EndoA3 BAR in A3 BAR-A2 SH3 chimera | AAGCCCCGGCCCCGG | |
| 5' for Lifeact EGFP in pBABE Hygro | CTCCTTCTCTAGGCGCCGGCCGGTACCATGGGCGTGGCCGACCTGATCAAGAAGTTC | |
| 3' for Lifeact EGFP in pBABE Hygro | GGGTCGACCACTGTGCTGGCGAATTCTTACTTGTACAGCTCGTCCATGCCG | |
| 5' for EndoA3ΔN truncation | CTCTAGGCGCCGGCCGGATCCATGATAAGTGGTGCCGAAGGAACG | |
| 5' for K171N mutant of EndoA3 | CGCCTGGATTATGATTATAACAAGCGGCGGGTAGGTAAG | |
| 3' for K171N mutant of EndoA3 | CTTACCTACCCGCCGCTTGTTATAATCATAATCCAGGCG | |
| 5' for R174L of EndoA3 | TATGATTATAAAAAGCGGCTGGTAGGTAAGATCCCCGAG | |
| 3' for R174L of EndoA3 | CTCGGGGATCTTACCTACCAGCCGCTTTTTATAATCATA | |
| Recombinant DNA | ||
| Plasmid: mEndoA3-mCherry in pBABE Puro | Laboratory of Jihong Bai | BJP-R82.8 |
| Plasmid: mEndoA3ΔN-mCherry in pBABE Puro | Laboratory of Jihong Bai | BJP-R81.1 |
| Plasmid: Chimera mEndoA3 N-BAR::mEndoA2 SH3::3xFlag in pBABE PURO | Laboratory of Jihong Bai | BJP-R73 |
| Plasmid: Chimera mEndoA2 N-BAR::mEndoA3 SH3::3xFlag in pBABE PURO | Laboratory of Jihong Bai | BJP-R72 |
| Plasmid: mEndoA1::3xFlag in pBABE Puro | Laboratory of Jihong Bai | BJP-R55 |
| Plasmid: mEndoA2::3xFlag in pBABE Puro | Laboratory of Jihong Bai | BJP-R56 |
| Software and Algorithms | ||
| FIJI | NIH | https://fiji.sc |
| FlowJo | Tree Star | www.flowjo.com |
| Tissue Fax | Tissuegnostics | www.tissuegnostics.com/ |
| Zen | Zeiss | www.zeiss.com |
| Fluoview (FV10-ASW) | Olympus Corporation | http://www.olympusamerica.com/ |
| Metamorph Microscopy and Image Analysis Software | Molecular Devices | www.moleculardevices.com |
| Other | ||
| Human Tissue Microarray | US Biomax | Cat#: BC05118C |