Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Drosophila melanogaster) |
Double Header Jump Starter phase 0 on chromosome II |
This study | Fly strain containing DH flanked by LoxP sites |
|
| Genetic reagent (D. melanogaster) |
Double Header Jump Starter phase one on chromosome II |
This study | Fly strain containing DH flanked by LoxP sites |
|
| Genetic reagent (D. melanogaster) |
Double Header Jump Starter phase two on chromosome II |
This study | Fly strain containing DH flanked by LoxP sites |
|
| Genetic reagent (D. melanogaster) |
Double Header Jump Starter phase 0 on chromosome III |
This study | Fly strain containing DH flanked by LoxP sites |
|
| Genetic reagent (D. melanogaster) |
Double Header Jump Starter phase one on chromosome III |
This study | Fly strain containing DH flanked by LoxP sites |
|
| Genetic reagent (D. melanogaster) |
Double Header Jump Starter phase two on chromosome III |
This study | Fly strain containing DH flanked by LoxP sites |
|
| Genetic reagent (D. melanogaster) |
MI01487 (kibra) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0040175; RRID:BDSC_40175 |
|
| Genetic reagent (D. melanogaster) |
MI05208 [5-HT2B (5-hydroxytryptamine receptor 2B)] |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0042994; RRID:BDSC_42994 |
|
| Genetic reagent (D. melanogaster) |
MI06794 [Lgr4(Leucine-rich repeat-containing G protein-coupled receptor 4)] |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0042179: RRID:BDSC_42179 |
|
| Genetic reagent (D. melanogaster) |
MI06872 (CG34383) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0041111; RRID:BDSC_41111 |
|
| Genetic reagent (D. melanogaster) |
MI08614 [Dgk (Diacyl glycerol kinase)] |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0044991; RRID:BDSC_44991 |
|
| Genetic reagent (D. melanogaster) |
MI11741 (CG12206) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0056687; RRID:BDSC_56687 |
|
| Genetic reagent (D. melanogaster) |
MI15073 (CG9132) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0059739; RRID:BDSC_59739 |
|
| Genetic reagent (D. melanogaster) |
MI02926 (Pits) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0036165; RRID:BDSC_36165 |
|
| Genetic reagent (D. melanogaster) |
MI00805 (CG6966) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0034113; RRID:BDSC_34113 |
|
| Genetic reagent (D. melanogaster) |
MI12643 (fz) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0058645; RRID:BDSC_58645 |
|
| Genetic reagent (D. melanogaster) |
MI05871 (Doa) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0043880; RRID:BDSC_43880 |
|
| Genetic reagent (D. melanogaster) |
MI08818 (qless) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0051110; RRID:BDSC_51110 |
|
| Genetic reagent (D. melanogaster) |
MI06179 (DCX-EMAP) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0043047; RRID:BDSC_43047 |
|
| Genetic reagent (D. melanogaster) |
MI14396 (CG6293) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0059511; RRID:BDSC_59511 |
|
| Genetic reagent (D. melanogaster) |
MI00445 (Nlg3) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0031005; RRID:BDSC_31005 |
|
| Genetic reagent (D. melanogaster) |
MI09222 (CG1578) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0051263; RRID:BDSC_51263 |
|
| Genetic reagent (D. melanogaster) |
MI00494 (wnd) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015b |
flybaseID# _FBst0031028; RRID:BDSC_31028 |
|
| Genetic reagent (D. melanogaster) |
MI02915 (Ask1) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0036163; RRID:BDSC_36163 |
|
| Genetic reagent (D. melanogaster) |
MI03136 (LPCAT) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0036425; RRID:BDSC_36425 |
|
| Gnetic reagent (D. melanogaster) |
MI10071 (Trpl) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0053455; RRID:BDSC_53455 |
|
| Genetic reagent (D. melanogaster) |
MI09900 (Sap47) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID# _FBst0053794; RRID:BDSC_53794 |
|
| Genetic reagent (D. melanogaster) |
MI01646 (CG1815) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0035948; RRID:BDSC_35948 |
|
| Genetic reagent (D. melanogaster) |
MI04010 (Tbh) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0056660; RRID:BDSC_56660 |
|
| Genetic reagent (D. melanogaster) |
MI13728 (CG17841) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0059189; RRID:BDSC_59189 |
|
| Genetic reagent (D. melanogaster) |
MI05741 (CG1632) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0042106; RRID:BDSC_42106 |
|
| Genetic reagent (D. melanogaster) |
MI10889 (CG17167) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0056092; RRID:BDSC_56092 |
|
| Genetic reagent (D. melanogaster) |
MI00986 (CG32698) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0035095; RRID:BDSC_35095 |
|
| Genetic reagent (D. melanogaster) |
MI15214 (CG13375) |
Venken et al. (2011a); Nagarkar-Jaiswal et al., 2015a |
flybaseID#_ FBst0060995; RRID:BDSC_60995 |
|
| Genetic reagent (D. melanogaster) |
Nmnatywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the geneNmnat |
|
| Genetic reagent (D. melanogaster) |
Stub1ywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene Stub1 |
|
| Genetic reagent (D. melanogaster) |
Ubqnywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene Ubqn |
|
| Genetic reagent (D. melanogaster) |
Itp-r83Aywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene Itp-r83 |
|
| Genetic reagent (D. melanogaster) |
CG18769ywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene CG18769 |
|
| Genetic reagent (D. melanogaster) |
CG13390ywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene CG13390 |
|
| Genetic reagent (D. melanogaster) |
Med27ywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene Med27 |
|
| Genetic reagent (D. melanogaster) |
CG11679ywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene CG11679 |
|
| Genetic reagent (D. melanogaster) |
amxywing2+ | This study | fly strain carrying the ywing2+ dominant marker replacing the gene amx |
|
| Genetic reagent (D. melanogaster) |
Nmnat::GFP::NmnatWT | This study | fly strain carrying the Nmnat gene with S(GSS)4... EGFP coding sequence… (GSS)4 integrated internally into the protein between 3R:24945353 and 3R:24945353 |
|
| Genetic reagent (D. melanogaster) |
Nmnat::GFP::NmnatW129G | This study | fly strain carrying the Nmnat gene with S(GSS)4...EGFP coding sequence…(GSS)4 integrated internally into the protein between 3R: 24945353 and 3R:24945353 and bearing a mutation producing W192G |
|
| Genetic reagent (D. melanogaster) |
Nmnat::GFP::NmnatΔ251…257 | This study | fly strain carrying the Nmnat gene with S(GSS)4...EGFP coding sequence…(GSS)4 integrated internally into the protein between 3R: 24945353 and 3R:24945353 and bearing a deletion removing amino acids 251…257 |
|
| Genetic reagent (D. melanogaster) |
Nmnat::GFP::NmnatC344S, C345S | This study | fly strain carrying the Nmnat gene with S(GSS)4... EGFP coding sequence… (GSS)4 integrated internally into the protein between 3R: 24945353 and 3R:24945353 and bearing a mutation producing C344S, C345S |
|
| Recombinant DNA reagent |
Double Header | This study | Recombination Mediated Cassette Exchange donor plasmid containing SA-T2A -GAL4-polyA and SA-GFP-SD in the opposite orientation |
|
| Recombinant DNA reagent |
pattB ywing2+ | This study | Vector for φC31 integrated transgenesis that expresses the yellow gene product in the wings |
|
| Recombinant DNA reagent |
pattB ybody+ | This study | Vector for φC31 integrated transgenesis that expresses the yellow gene product in the body |
|
| Recombinant DNA reagent |
p{ywing2+} | This study | Donor vector compatible with Golden Gate cloning carrying the yellow wing2 + dominant marker flanked by nucleotides ‘GG’ and ‘CC’ upstream and downstream, respectively |
|
| Recombinant DNA reagent |
p{ybody+} | This study | Donor vector compatible with Golden Gate cloning carrying the yellow body dominant marker flanked by nucleotides ‘GG’ and ‘C C’ upstream and downstream, respectively |
|
| Recombinant DNA reagent |
p{EGFP Donor} | This study | Donor vector compatible with Golden Gate cloning carrying the EGFP coding sequence flanked by (GSS) linker sequences |
|
| Recombinant DNA reagent |
p{mCherry Donor} | This study | Donor vector compatible with Golden Gate cloning carrying the mCherry coding sequence flanked by (GSS) linker sequences |
|
| Recombinant DNA reagent |
p{T2a-GAL4 Donor}} | This study | Donor vector compatible with Golden Gate cloning carrying the T2a viral peptide sequence in frame with the GAL4 transcription factor coding sequence |
|
| Recombinant DNA reagent |
p{T2a-GAL4-PolyA Donor}} | This study | Donor vector compatible with Golden Gate cloning carrying the T2a viral peptide sequence in frame with the GAL4 transcription factor coding sequence terminating in the SV40 transcriptional terminator |
|
| Recombinant DNA reagent |
pCFD3-dU6:3gRNA | Port et al. (2014) | Addgene_ plasmid_#49410 |
|
| Recombinant DNA reagent |
Double Header | This study | Recombination Mediated Cassette Exchange donor plasmid containing SA-T2A-GAL4-polyA and SA-GFP-SD in the opposite orientation |
|
| Antibody | anti-GFP antibody conjugated with FITC |
Abcam | RRID: AB_305635 | used at 1:500 |
| Antibody | anti-GFP | Invitrogen | Cat#_ A11122 | used at 1:500 |