Table 2. Summary statistics for cassette swapping experiments.
| Construct | Injected genotype: | No. embryos injected | No. fertile adults | No vials with y- flies | % of y- flies confirmed positive |
|---|---|---|---|---|---|
| Nmnat:GFP:Nmnat wt #1 | y1 M{nos-Cas9.P}ZH-2A w*;+; ywing2+ ΔNmnat/TM6B | 514 | 7 | 4 | 6% |
| Nmnat:GFP:Nmnat wt #2 | y1 M{nos-Cas9.P}ZH-2A w*;+; ywing2+ ΔNmnat/TM6B | 607 | 16 | 5 | 21% |
| Nmnat:GFP:NmnatW129G #1 | y1 M{nos-Cas9.P}ZH-2A w*;+; ywing2+ ΔNmnat/TM6B | 653 | 0 | - | - |
| Nmnat:GFP:NmnatW129G #2 | y1 M{nos-Cas9.P}ZH-2A w*;3 KB NMNAT GRC; ywing2+ ΔNmnat | 418 | 31 | 3 | 55% |
| Nmnat:GFP:NmnatΔ251…257 | y1 M{nos-Cas9.P}ZH-2A w*;3 KB NMNAT GRC; ywing2+ ΔNmnat | 496 | 29 | 11 | 24% |
| Nmnat:GFP:NmnatC344S, C345S | y1 M{nos-Cas9.P}ZH-2A w*;3 KB NMNAT GRC; ywing2+ ΔNmnat | 386 | 30 | 12 | 14% |
| Stub1:GFP | y1 M{nos-Cas9.P}ZH-2A w*; ywing2+ ΔStub1 | 235 | 62 | 2 | 66% |
| CG11679:Flag | ywing2+ ΔCG11679/FM7 Kgal4,UAS GFP;+/+; attP2(y-){nos-Cas9 | 976 | 12* | 3 | 33% |
| Med27:flag | y1 M{nos-Cas9.P}ZH-2A w*;+; ywing2+ ΔMed27 | 833 | 17 | 3 | 29% |
| Amx:GFP | ywing2+ Δamx;+/+; attP2(y-){nos-Cas9 | 648 | 34 | 0 | - |
*excluding FM7 homozygotes and hemizygotes.