Figure 2. LNC CRYBG3 prevents tumor growth and reduces tumor cell metastasis and invasion.

(A) The tumor volume was detected for each group on 30^th day. (B) Calculate the theoretically time requirement for the tumor to grow to 100 mm³. The tumor formation frequency was calculated as described in methods (n = 10 for each group). (C) Xenografted tumor grows curve after overexpressed LNC CRYBG3 or negative control. The injection was begun on the time when xenografted tumor get 100 mm³ twice a week for 1 month (n=10 for each group). (D) The xenografed tumors obtained after 8 times injection (n=10 for each group). (E) The weight of xenografed tumors from different groups was compared (n=10 for each group). (F) Representative H&E staining of transplanted lung tumor tissues are shown. (G) In vivo cells metastasis experiment (n=5 for each group). (H&I) Up-regulation of LNC CRYBG3 reduced the metastatic potential of A549 cells. Images showing trans-well invasion of the A549 cells. The three images in the upper panel show invasion of A549 cells in control or overexpressed with negative control or LNC CRYBG3. The images in the bottom two panels show the short hairpin of LNC CRYBG3 RNA expressed cells overexpressed with LNC NC or LNC CRYBG3. (J&K) Up-regulation of LNC CRYBG3 influences the invasive property of A549 cells. Images showing matrigel invasion of the A549 cells. Data represent the mean ± SE of three independent biological experiments.