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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Nutr Biochem. 2018 May 12;58:59–70. doi: 10.1016/j.jnutbio.2018.04.018

Fig. 2.

Fig. 2

Genistein stimulation of cAMP production was mediated via GPR30 in β-cells. (A) protein expression of GPR30 in insulin-secreting cells and islets as determined by western blot (H=human, M=mouse). (B) Intracellular cAMP levels in INS1 cells preincubated with vehicle (V) or GPR30 antagonist G15 (5 μM) for 10 min before stimulation with genistein (G, 5 μM) for 20 min. (C) Knockdown of GPR30 protein expression in INS1 cells transfected with GPR30 siRNA (I) or scrambled siRNA (S). Images shown were from two independent transfection experiments. (D) Intracellular cAMP levels in GPR30 siRNA (I) or scrambled siRNA (S) transfected cells and exposed to 5 μM genistein or vehicle (V) for 20 min. (E) Intracellular cAMP levels in wild-type (WT) or GPR30−/− (KO) islets incubated with vehicle (V), genistein (G, 5 μM), or foskolin (F, 1 μM) for 20 min. (F) Western blot for GPR30 knockout in the islets. Cyclic AMP levels were normalized to the protein contents, and expressed as percentage of the control value. *, p<0.05 (n=3-4±SEM).