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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Pain. 2018 Sep;159(9):1802–1813. doi: 10.1097/j.pain.0000000000001277

Figure 5. TLQP-62 increases responses of high threshold dorsal horn neurons to moderate mechanical stimulation.

Figure 5

A) Experimental outline. Spinal single units recorded in vivo were tested for response to successive 10 s mechanical stimulation in the form of brush (B), pressure (Pr) and pinch (Pi) with a 10 s inter-stimulus-interval. Following three sets of baseline responses, TLQP-62 (50 nM) or vehicle was applied to the exposed spinal cord for 15 minutes, after which responses were assessed for up to 1 h. B) Histologically identified recording sites. C) Baseline responses of high threshold (HT) and wide dynamic range (WDR) units were not significantly different between TLQP-62 and vehicle exposed groups (P > 0.05; Fisher’s Exact test). The number of units of each type is shown in parentheses. D) The response of single units was deemed significantly altered if the fold change relative to baseline response was > 2SD of that observed in the vehicle group. A significantly larger proportion of HT but not WDR units exposed to TLQP-62 exhibited an increase in response to pressure, relative to controls; *=P<0.05 Fisher’s Exact test. E) Representative example of HT units exposed to saline (left) and TLQP-62 (right). Note the emergence of a pressure response following exposure to TLQP-62.