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. 2018 Aug 16;9:3281. doi: 10.1038/s41467-018-05799-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — MoNaLISA nanoscale imaging. a Super-resolved image of endogenous vimentin-rsEGFP2 in comparison to the related wide-field image (inset). Scale bar 5 μm. b and c Magnified regions showing wide-field, enhanced confocal, and MoNaLISA images. Scale bar 500 nm. d Representative normalized intensity profiles (four lines averaged) measured across the filaments marked with white arrowheads no. 1 in b. Gaussian fits of the data show a FWHM of 55 ± 5 nm, 180 ± 10 nm, and 335 ± 45 nm for imaging in MoNaLISA, enhanced confocal, and wide-field mode, respectively (±s.e.m.). e Normalized intensity profiles (four lines averaged) measured across the filaments marked with white arrowheads no. 2 in b. The Gaussian fit for the two filaments shows a FWHM of 50 ± 5 nm and 85 ± 10 nm, respectively, and a separation of 85 ± 5 nm (±s.e.m.). f Histogram of 70 independent filament widths measured in image a, the mean value is 55 ± 10 nm (mean ± s.d.). g Normalized intensity profile measured in f across the white arrows. The MoNaLISA data shows five distinct filaments that cannot be resolved using confocal or widefield imaging