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. 2018 Aug 16;9:3281. doi: 10.1038/s41467-018-05799-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — MoNaLISA time-lapse imaging. a Nanoscale imaging of epithelial cells expressing LifeAct-rsEGFP2 compared to the wide-field image (inset). Scale bar, 10 μm. b Magnified regions I and II unravel the dynamics of growing small filopodia observed over 3 min for a total of 45 frames, each acquired in 3.9 s. Scale bar, 1 μm. c Living hippocampal neurons infected with LifeAct-DronpaM159T showing the actin arrangements in dendrites and dendritic spines. Scale bar, 10 μm. d Magnified region III highlights the recruitment of actin molecules into a spine, which slowly enlarge its head into a cup-shape across 19 frames (left arrow), each acquired in 3.4 s. Scale bar, 1 μm. e Dynamics of dendritic filopodia emerging from actin densities in the dendritic shaft in neuron at 8 DIV. The arrows highlight the actin dynamics and redistribution. The graph shows the elongation over time of the filopodia 2 and reveals nanosized increments across time. Each line profile was averaged over a 100 nm width. f Nanoscale imaging of the mitochondria outer membrane labeled with rsEGFP2-Omp25. The mitochondrial volumetric dynamics are recorded continuously over seconds. The axial information is color-coded. Single frame recording 0.83 s, volume 3.3 s. Scale bar, 1 μm