Fig. 4.

LoAD system can affect various genome editing outcomes. a Schematic of the enhancement of short deletion with MS2-Trex2 LoADing. b Frequency of short deletion at the LMNB1 locus detected by RFLP analysis. Data are expressed as means ± s.e.m. (n = 3). *P < 0.05 (Student’s t-test). c Frequency of short deletion at the RPL11 locus detected by RFLP analysis. Data are expressed as means ± s.e.m. (n = 3). **P < 0.01 (Student’s t-test). d Subcloned sequencing analysis revealed that the LoADing of MS2-Trex2 resulted in high-frequency deletions. e Schematic of the enhancement of independent mutations and the suppression of chromosomal deletion with MS2-Trex2 LoADing. f Pseudo-gel image of the out–out PCR products at APC locus 1, illustrated in Supplementary Figure 19a. Black and white arrowheads indicate the expected positions of the wild type and chromosomally deleted PCR products, respectively. g Percentages of chromosomally deleted alleles among all the out–out PCR products at APC locus 1. Data are expressed as means ± s.e.m. (n = 3). **P < 0.01 (Student’s t-test). N.S., not significant. h Percentages of independently mutated alleles among the full-length PCR products indicated by the black arrowhead in Fig. 4f. The mutations were detected by genomic cleavage detection assay³⁶. Pseudo-gel image of this assay is shown in Supplementary Figure 19b. Data are expressed as means ± s.e.m. (n = 3). **P < 0.01 (Student’s t-test). i Schematic of the enhancement of ssODN knock-in mediated by single-strand template repair (SSTR) with MS2-CtIP LoADing. j Efficiencies of ssODN knock-in detected by RFLP analysis, related to Supplementary Figure 20. Data are expressed as means ± s.e.m. (n = 3). **P < 0.01 (Student’s t-test). k Schematic of the enhancement of plasmid knock-in mediated by homologous recombination (HR) with MS2-CtIP LoADing. l Efficiencies of HR-mediated knock-in, related to Supplementary Figure 21. The knock-in rate was calculated as the percentages of knock-in clones among the total number of clones