Fig. 1.

Workflow of the global genome mining of CNRP BGCs. a 7395 complete or draft bacterial genomes were downloaded from GenBank and subjected to stand-alone antiSMASH for BGC analysis and NRP sequence prediction, leading to the identification of 29,461 NRPS BGCs, in which 11,286 BGCs encoded NRPs with positively charged residues. b Selected peptide sequences of cationic peptides (numbering 807) were submitted to the EFI–Enzyme Similarity Tool (EFI–EST) for constructing the peptide similarity network. c The CNRPs with desirable chemical features were obtained by conventional fermentation-based isolation