Table 3.

KRAS mutational analysis of total DNA extract from CTC isolates from pancreatic cancer patient blood samples^a

IDa	CTCsb	PBMCsc	KRAS mutation	Mutated KRAS genes (copies per µL)d
Patient 1	68	1170	G12D	0.94
Patient 2	87	1885	G12D	1.03
Patient 3	90	1075	G12V	1.05
Patient 4	107	1072	G12D	1.13

^aBlood samples derive from the same pancreatic cancer patients as in Fig. 4a

^bCTCs were defined as CK+, DAPI+, CD45−

^cPBMCs were defined as CK−, DAPI+, CD45+

^dFor the analysis of each patient sample, 100 PDAC cells were spiked into 5000 PBMCs prior to the DNA extraction procedure and run in parallel as a positive control, resulting in values between 0.93 and 0.94 copies of mutated KRAS genes per microliter