Table 2.

Primers used in RT-qPCR assays.

Gene	Sense	Primer sequence (5′ → 3′)	Product lenght (bp)	Melting temperature (°C)
GAPDH	F	TGATGACATCAAGAAGGTGGTGAAG	240	62
	R	TCCTTGGAGGCCATGTAGGCCAT
IRE1α	F	TCACAAAGTGGAAGTACCCG	99	57
	R	AGGCATAGAGGCTGGTAG
PERK	F	AGACGATGAGACAGAGTTGCG	156	58
	R	TTGCTAAGGCTGGATGACACC
ATF6	F	TCAAGCACCTGGAGTTCTG	200	57
	R	GTCTCCTTAGCACAGCAATATC
sXBP1	F	CTGAGTCCGCAGCAGGTG	221	57
	R	GGAGGCTGGTAAGGAACTGG
CHOP	F	TTAAGTCTAAGGCACTGAGCGTATC	135	55
	R	TGCTTTCAGGTGTGGTGATG
TXNIP	F	GACCGCCCGAGCCAGCCAAC	156	62
	R	AGAGACAGACACCCGCCCATCAGG
NLRP3	F	TCAGACAGAGAAGGCAGACC	194	58
	R	GGCATATCACAGTGGGATTCG
IL-1β	F	TGATGGCTTATTACAGTGGCAATG	140	62
	R	GTAGTGGTGGTCGGAGATTCG

Oligonucleotide primers were designed using the online tool PrimerBlast (NCBI NIH, USA).