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. 2018 Aug 16;8:12284. doi: 10.1038/s41598-018-30682-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GNMT was suppressed by miR-224 in liver cancer cell lines. (A) The expression levels of miR-224 and GNMT in three liver cancer cell lines and 78 pairs of HCC patients were measured by RT-qPCR. T, HCC tissue, TA, tumor-adjacent liver tissue. The expression levels of miR-224 and GNMT were measured in HCC cell lines transfected with (B–D) 224-mimic and (E) 224-inhibitor, control group NC or NC inhibitor by qPCR (upper panel) and western blotting (bottom panel). The GNMT and miR-224 expression levels of each sample were normalized with the level of TBP and RNU48. β-actin was used as internal control for western blotting. *P < 0.05, **P < 0.01, ***P < 0.001.