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. 2018 Aug 16;8:12284. doi: 10.1038/s41598-018-30682-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

miR-224 induced tumor growth of HCC cells through regulation of GNMT. The effect of lentivirus expressing miR-224 and GNMT on Huh7 cells growth in vitro and in vivo. (A) The GNMT, miR-224 and GNMT/miR224 transcript levels in Huh7 infected with lentivirus expressing miR-224 and GNMT were measured by qPCR (upper panel). (B) The colony formation was quantified by staining crystal violet. (C) A subcutaneous xenograft of lentivirus expressing eGFP, GNMT, miR-224 and GNMT/miR-224 of Huh7 was injected into NOD/SCID mice (N = 6, per group). The length of formed tumors was recorded in centimeters. (D) The tumor growth curve was measured every 7 days for 35 days after inoculation. GNMT protein expression examined by western blot analysis using anti-GNMT and ant-α-tubulin antibodies (bottom panel). *P < 0.05, **P < 0.01, ***P < 0.001.