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. 2018 Aug 16;8:12284. doi: 10.1038/s41598-018-30682-5

Figure 5.

Figure 5

The mRNA expression of GNMT was negatively correlated with miR-224 in HBV-associated HCC specimens and transgenic HBx mice. (A) The expression levels of GNMT and miR-224 were measured by RT-qPCR in 40 pairs of HBV-associated HCC, 20 pairs of HCV-associated HCC and 18 pairs of HCC patients without hepatitis viral infection. The relative expression fold of GNMT and miR-224 were normalized to TBP and RNU48 RNA, respectively. (B) Pearson correlation analysis for miR-224 and GNMT expressions in 40 paired HBV-associated HCC, 20 paired HCV-associated HCC and 18 paired HCC patients without hepatitis viral infection. T, HCC tissue, TA, tumor-adjacent liver tissue. (C) The expression levels of GNMT and miR-224 measured by qPCR in liver without tumor were from 1.5-, 6~8-, 12~14-month-old wild type (WT: 1.5 M, n = 3; 6–8 M, n = 6; 12–14 M, n = 12, total n = 21) and transgenic HBx mice (HBx: 1.5 M, n = 5; 6–8 M, n = 6; 12–14 M,n = 8), and in liver with tumor was from 16-month-old transgenic HBx mice (16M-T, n = 6). The relative expression fold of GNMT and miR-224 were normalized to mouse β-2-microglobulin (β2m) and sno202 RNA, respectively. (D) Pearson correlation analysis for GNMT and miR-224 expressions in WT mice liver or HBx mice liver with/without tumor (HBx and HBx-T). *P < 0.05, **P < 0.01, ***P < 0.001; NS, Not significant.