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. 2018 Aug 16;8:12271. doi: 10.1038/s41598-018-29924-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Release of CD81-positive extracellular vesicles during liver regeneration. Exosomes were isolated from serum samples using ExoQuick and exosome lysates were analysed by Western blotting using an antibody against CD81 (A, upper panel). The protein band corresponding to CD81 is indicated by an arrowhead. Protein loading was controlled by coomassie blue staining of gels (A, lower panel). For each time point, samples from six individual wildtype mice are shown. Full length gels are depicted. Expression of CD81 was quantified by densitometric analyses (B). *P < 0.05, **P < 0.01 (two-tailed unpaired Student’s t-test or the Mann-Whitney U test).