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. 2018 Aug 16;8:12252. doi: 10.1038/s41598-018-29708-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-342-3p is regulated by ER and its exogenous expression down-modulates oncogenic pathways in TN cells. (a) MCF7 cell line transfected with siRNA-mediated sequences against ER. Evaluation of the down-modulation of ER by an immunoblotting analysis after 48 and 72 hr of siRNA transfection. Densitometric analysis indicated as percentage above the blot (the blot is representative of 3 independent experiments). Real-time qRT-PCR evaluation in MCF7 cells transfected with ER siRNAs at 48 and 72 hr post-transfection of (b) EVL, (c) pre-miR-342 and (d) miR-342-3p. Cell growth rate evaluation by SRB assay after 144 h in (e) BT549, (f) SUM149, (g) SUM159, (h) MDAMB157 and (i) MDAMB468. (j) Cell viability evaluated by MTS assay at 48 h post-transfection, (k) cell migration evaluated by a transwell assay on TNBC cells transfected with miR-342-3p precursor or control for 48 h. Results of three independent experiments with three technical replicates are represented as the geometric mean +/− standard deviation error.