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. 2018 Aug 16;8:12252. doi: 10.1038/s41598-018-29708-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exogenous expression of miR-342-3p disrupts extra and intracellular lactate concentrations, and glucose levels. (a) Evaluation of exogenous lactate (10 mM) consumption of MDAMB468 cells transfected with miR-342-3p precursor quantified by an enzymatic colorimetric assay (cells were cultured in low glucose medium). Enzymatic colorimetric assay of endogenous lactate concentration of MDAMB468 cells transfected with miR-342-3p precursor or control in (b) extracellular (medium) or (c) intracellular compartments. (d) Evaluation by immunoblotting analysis of HIF1A and GLUT1 protein expression at 24, 48 and 72 hr post-transfection of miR-342-3p precursor in MDAMB468 cells. Densitometric analysis is indicated as percentage above the blot. (e) qRT-PCR assay of MCT1, HIF1A, MCT4 and LDHA expression on MDAMB468 cells transfected with miR-342-3p precursor. Enzymatic colorimetric assay of endogenous glucose concentration of MDAMB468 cells transfected with miR-342-3p precursor or control in (f) extracellular or (g) intracellular compartments. Glycolytic index of cells exogenously transfected with miR-342-3p compared to control in (h) extracellular and (i) intracellular compartments. The plots are representative of three independent experiments with three technical replicates, each summarized as the geometric mean +/− standard deviation error.