Fig. 5.

PfCRT mutations mediate different impacts on P. falciparum growth rates in vitro. pfcrt-edited lines were co-cultured for 20 days with a GFP-expressing Dd2 line and cultures were sampled every 2–3 days by flow cytometry. Experiments were conducted on two separate occasions in triplicate, and the mean ± SEM percentage of GFP⁺ parasites were plotted over time. Values below the 50% dashed line indicate that a line was fitter than GFP⁺ parasites. a Results with PfCRT SNPs added to the Dd2 PfCRT haplotype in recombinant Dd2 parasites. Only the M343L mutation had comparable fitness to the Dd2 allele, whereas the two other variants (F145I and G353V) were less fit. b Results of Cambodian isolates and isogenic lines with novel PfCRT SNPs removed, showing improved fitness in the lines lacking H97Y or M343L. Raw data are presented in Supplementary Table 12