Table 1.
Haplotypes of pfcrt-edited and control P. falciparum lines
| PfCRT haplotype at listed position | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Parasite line | Edited pfcrt | 72 | 74 | 75 | 76 | 97 | 144 | 145 | 148 | 194 | 220 | 271 | 326 | 333 | 343 | 353 | 356 | 371 |
| Dd2 | No | C | I | E | T | H | A | F | L | I | S | E | S | T | M | G | T | I |
| Dd2Dd2 crt | Yes | C | I | E | T | H | A | F | L | I | S | E | S | T | M | G | T | I |
| Dd2Dd2 crt F145I | Yes | C | I | E | T | H | A | I | L | I | S | E | S | T | M | G | T | I |
| Dd2Dd2 crt M343L | Yes | C | I | E | T | H | A | F | L | I | S | E | S | T | L | G | T | I |
| Dd2Dd2 crt G353V | Yes | C | I | E | T | H | A | F | L | I | S | E | S | T | M | V | T | I |
| PH1008-C | No | C | I | E | T | H | A | F | L | I | S | E | S | T | L | G | T | I |
| PH1008-CDd2 crt | Yes | C | I | E | T | H | A | F | L | I | S | E | S | T | M | G | T | I |
| PH1263-C | No | C | I | E | T | Y | A | F | L | I | S | E | S | T | M | G | T | I |
| PH1263-CDd2 crt | Yes | C | I | E | T | H | A | F | L | I | S | E | S | T | M | G | T | I |
| Cam734 | No | C | I | D | T | H | F | F | I | T | S | E | N | S | M | G | I | R |
| GB4 | No | C | I | E | T | H | A | F | L | I | S | E | N | T | M | G | I | I |
| 3D7 | No | C | M | N | K | H | A | F | L | I | A | Q | N | T | M | G | I | R |
pfcrt was edited using customized zinc finger-nucleases, and clones were generated by limiting dilution. Differences from the 3D7 wild-type allele are shown in italics for Dd2 and bold for Cam734 mutations. Underline indicates mutations identified from piperaquine-resistant field isolates and tested herein by gene editing