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. 2018 Aug 10;9:900. doi: 10.3389/fphar.2018.00900

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Copyright © 2018 Yang, Guo, Dong, Sheng, Wang, Yu, Wang and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HG induces U937 cell migration and inflammatory cytokine expression through miR-374a. (A–C) ELISA analysis of IL-6 and -18 and TNF-α in the culture supernatant. ^∗P < 0.05 vs. NG group (n = 3). (D) Quantitative analysis of U937 cell migration in different groups. ^∗P < 0.05 vs. NG or HO group (n = 3). (E,F) qRT-PCR analysis of miR-374a and MCP-1 mRNA. ^∗P < 0.05 vs. negative control (miR-NC) group (n = 3). (G) Quantification of U937 cells with the transwell migration assay. ^∗P < 0.05 vs. miR-NC group (n = 3). (H–J) After the transwell migration assay, IL-6 and -18 and TNF-α levels in the culture supernatant of HK2 cells transfected with a miR-374a mimic or inhibitor were monitored. ^∗P < 0.05 vs. miR-NC group (n = 3).