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. 2018 Aug 10;9:900. doi: 10.3389/fphar.2018.00900

FIGURE 4.

FIGURE 4

Overexpression of MCP-1 antagonizes the effect of miR-374a mimic. (A) MCP-1 protein level in HK2 cells after co-transfection of pcDNA3.1-MCP-1, as determined by western blotting. P < 0.05. (B) Numbers of migrating U937 cells in the co-culture system with or without MCP-1 overexpression under HG conditions. (C) Detection of IL-6 and -18 and TNF-α by ELISA in the co-culture system with or without MCP-1 overexpression. P < 0.05.