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. 2018 Aug 10;9:1833. doi: 10.3389/fimmu.2018.01833

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Copyright © 2018 Kopecki, Stevens, Chong, Yang and Cowin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Over-expression of Flii produces an altered autoantibody profile in murine OVA-induced atopic dermatitis (AD) skin-like disease. (A) Representative images of IF microscopy staining patterns produced by mouse autoreactive IgG (red) and DAPI nuclear counterstaining (blue) in primary mouse keratinocytes. Magnification 20×; scale bar 50 µm. Antibodies from OVA-induced AD skin-like lesions of Flii^+/− and WT mice producing a predominantly cytoplasmic (white arrow) and perinuclear (green arrow) staining patterns, while antibodies from OVA-induced AD skin-like lesions of Flii^Tg/Tg mice produce a strong cytoplasmic, perinuclear (green arrow), and nuclear (black arrow) staining patterns. (B) Western blot analysis of murine keratinocyte proteins probed with pooled sera from OVA-induced AD-like mice (2–3 mice per lane); black arrows represent regions of autoreactivity. (C) Autoreactivity summary of Western blot results from murine keratinocyte lysate probed with sera from individual mice.