Table 3.

Differently expressed genes annotated “Androgen Signaling” pathway.

Gene Name	Gene Symbol	GC from PDF mean FPKM value	p-value	GCs from PMF FPKM value	p-value	Significance, paired t-test	Fold-change down
Protein Kinase C Iota	PRKCI	4,140	0,027	1,999	0,410	0,226	2,071
RNA Polymerase II Subunit D	POLR2D	2,508	0,186	1,236	0,423	0,460	2,029
G Protein Subunit Beta 5	GNB5	2,043	0,211	0,912	0,193	0,426	2,242
Protein Kinase C Beta	PRKCB	2,157	0,112	1,239	0,236	0,010	1,741

Differently expressed genes identified by comparing transcriptomes of the granulosa cells from primordial follicles vs. granulosa cells from primary follicles. Four genes were significantly downregulated during the primordial to primary follicle transition. Significance: fold-change >2 and/or paired t-test significance (p < 0.05) between the two FPKM mean values.