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. 2018 Aug 10;9:1856. doi: 10.3389/fimmu.2018.01856

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Copyright © 2018 Machelart, Potemberg, Van Maele, Demars, Lagneaux, De Trez, Sabatel, Bureau, De Prins, Percier, Denis, Jurion, Romano, Vanderwinden, Letesson and Muraille.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of T lymphocyte deficiency on Alt-induced Brucella susceptibility. Wild-type, γδTCR^−/−, TAP1^−/−, and MHCII^−/− C57BL/6 mice received repeated i.n. administration of phosphate-buffered saline or Alt before i.n. infection with 2 × 10⁴ CFU of mCherry-Brucella melitensis. The mice were sacrificed at 12 days post infection. (A) The data represent the number of eosinophils recruited (SIGLEC-F⁺ CD11c⁻ CD101⁺) × 10⁵ per lung from each group as determined by flow cytometry. (B) The data represent the number of CFU/gr of lung at 12 days post infection from each group. Horizontal gray lines represent the medians. n denotes the number of mice used for each lineage. These results are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.