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. 2018 Aug 10;9:1814. doi: 10.3389/fimmu.2018.01814

Table 2.

Plasmids used in this study.

Plasmid name Use Source
pSAG1-Cas9-sgUPRT Template for gene-specific CRISPR plasmid construction Addgene plasmid #54467
pSAG1-Cas9-sgLDH1 LDH1-specific CRISPR plasmid (28)
pSAG1-Cas9-sgOMPDC OMPDC-specific CRISPR plasmid This work
pOMPDC: loxp-DHFR*-loxp To replace OMPDC with loxp-DHFR*-loxp This work
pLDH1: loxp-DHFR*-loxp To replace LDH1 with loxp-DHFR*-loxp This work
pLDH1:DHFR Template for PUC19:LDH1-5UTR-3UTR amplification (28)
pDONR-G265-loxP-DHFR*-loxp Template for loxp-DHFR*-loxp amplification This work
pmin-Cre-eGFP To remove the selection marker DHFR* (38)