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. 2018 Aug 10;9:1849. doi: 10.3389/fmicb.2018.01849

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Copyright © 2018 Xia, Wu, Li, Xu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CydE binding sites and derepression analysis. (A) DNA footprinting. FAM-labeled DNA was the same as used in the EMSA and it was incubated with CydE in various amounts. Electropherograms indicated the protection pattern of DNA in different concentrations of CydE. (B) The promoter sequence of cydE. The red and purple highlighted sequences are binding sites 1 and 2, respectively. (C) EMSA for verification of the binding sites of CydE in the promoter region of cydE. The DNA of binding sites 1 and 2 sequences was synthesized and then incubated with CydE protein. The result showed that both the binding sites were shifted after adding CydE. (D) Derepression analysis. The free DNA band is shown with the addition of Na₂SO₄, while no similar phenomenon was observed with K₂CrO₄.