Figure 3.

Oridonin alters the expression of apoptosis-associated proteins in central neurocytoma cells. (A) The levels of Bcl-2, Bax, cleaved caspase-3 and cleaved PARP proteins in oridonin-treated cells and DMSO-treated control cells were detected by western blotting using respective specific antibodies. β-actin was used as the loading control. (B) Protein levels were quantified. **P<0.01. (C) The Bax/Bcl-2 ratio was calculated by dividing the value for Bax expression by the value for Bcl-2 expression. The assay was performed in triplicate. **P<0.01 vs. ctrl. Mean ± standard error of the mean. DMSO, dimethyl sulfoxide; Bcl-2, B-cell lymphoma 2; Bax, BCL-2-like protein 4; PARP, poly(ADP-ribose) polymerase 1; Ctrl, DMSO-treated control cells.