Figure 4.

Oridonin downregulates the Wnt/β-catenin signaling pathway. (A) The levels of β-catenin, cyclin D1 and c-Myc proteins in central neurocytoma cells that were treated with or without oridonin as evaluated by western blotting using respective specific antibodies. β-actin was detected as the internal reference. (B) Target protein expression normalized to β-actin expression is indicated by the bar graph. The assay was performed in triplicate. **P<0.01. Data are presented as the mean ± standard error of the mean. c-Myc, v-myc avian myelocytomatosis viral oncogene homolog; Ctrl, dimethyl sulfoxide-treated control cells.