Table I.
Primer sequences.
| Gene | Primer sequences (5′-3′) | Size (bp) |
|---|---|---|
| DTL (mRNA) | 490 | |
| Forward | CCTCTGTCCGATCCTCCAAA | |
| Reverse | AAAGATTTTCAGTCCCGCGG | |
| DTL (methyl) | 152 | |
| Forward | TTTTTTGTTCGATTTTTTAAAGACG | |
| Reverse | TAATAACCCTACAACTCTACCCGAA | |
| DUSP1 (mRNA) | 490 | |
| Forward | AGAATGTTCCTGACTCGGCA | |
| Reverse | AAGCAAAATCCAATCCCGGG | |
| DUSP1 (methyl) | 142 | |
| Forward | AGGCGAAAATATATAAGTTAAGCGA | |
| Reverse | AATACCGAATCAAAAACATTCTACG | |
| NFKBIA (mRNA) | 560 | |
| Forward | AGCGATGGGGTCTCACTATG | |
| Reverse | TCCAACAGCTTAGGTCAGGG | |
| NFKBIA (methyl) | 147 | |
| Forward | ATTTTAGTTTTTTAAGTAGGTGCGA | |
| Reverse | ATAAAACAAAAAAATCACTTACGTT | |
| SOCS2 (mRNA) | 630 | |
| Forward | GCTTGGGGTTAAATGGTGCA | |
| Reverse | AAGGGATGGGGCTCTTTCTC | |
| SOCS2 (methyl) | 181 | |
| Forward | GTATTGATTTTAAGGAAGGACGC | |
| Reverse | CCTACGAAAATAACTCCTCCG | |
| β-actin (mRNA) | 308 | |
| Forward | CATCTCTTGCTCGAAGTCCA | |
| Reverse | ATCATGTTTGAGACCTTCAACA | |
| β-actin (methyl) | 110 | |
| Forward | ATTATTATTGGTAATGAGCGGTTTC | |
| Reverse | TTCATAATAAAATTAAATATAATTTCGTA |
RT-qPCR, reverse transcription-quantitative polymerase chain reaction; MSP, methylation-specific polymerase chain reaction; methyl, methylation; DTL, denticleless protein homolog; DUSP1, dual specificity phosphatase 1; NFKBIA, nuclear factor of k light chain gene enhancer in B cells inhibitor, α; SOCS2, suppressor of cytokine signaling 2.