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. 2018 Jul 10;16(3):4033–4042. doi: 10.3892/ol.2018.9105

Figure 2.

Figure 2.

Biological characterization of 8305C-SP cells. (A) Proliferation rates of 8305C-SP and 8305C-MP cells determined using the MTT assay at 0, 24, 48, 72, 96 and 120 h. (B) Proliferation rates of BHT101-SP and BHT101-MP cells determined using the MTT assay at 0, 24, 48, 72, 96 and 120 h. Colony formation ability of SP and MP cells: (C) Colony formation was stained with Giemsa following culture for 14 days; (D) numbers of colonies formed in each well were counted using a microscope. Plating efficiency=(colony numbers/initial cell numbers) ×100%. (E) Mean time for xenograft formation. (F) Drug resistance to DDP was detected according to the inhibition of cell proliferation. Data are presented as the mean ± standard deviation. *P<0.05 (one-way analysis of variance). SP, side population; MP, main population; DDP, cisplatin.