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. 2018 Jul 4;16(3):3481–3488. doi: 10.3892/ol.2018.9077

Figure 4.

Figure 4.

Downregulation of Dock1 and Elmo1 reduces Rac1 activity and the expression of migration-associated proteins in MDA-MB-231 cells. (A) A Rac activity assay was performed to evaluate the Rac1 activity of MDA-MB-231 cells. (B) Reverse transcription-quantitative polymerase chain reaction and (C) western blot analysis assays were performed to measure the expression levels of FAK, Talin and Vinculin in MDA-MB-231 cells, MDA-MB-231 cells transfected with an empty vector, MDA-MB-231 cells transfected with si-Dock1 and MDA-MB-231 cells transfected with si-Elmo1. *P<0.05, **P<0.01 and ***P<0.001 vs. NC. Dock1, dedicator of cytokinesis 1; Elmo1, engulfment and cell motility 1; Rac1, Ras-related C3 botulinum toxin substrate 1; FAK, focal adhesion kinase; si, small interfering RNA; NC, negative control.