Figure 3.

TCDD treatment increased COX-2 and RANKL expressions on protein levels. Cells were treated with toluene (vehicle control) and 0.1, 1 and 10 nM TCDD for 24 and 72 h. (A) COX-2 and RANKL protein levels were measured by western blot analysis. (B) COX-2 protein levels at 24 and 72 h. Data are presented as the mean ± standard error of the mean (n=3). (C) RANKL protein levels at 24 and 72 h. *P<0.05 and **P<0.01 vs. the toluene-treated control (n=3). TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin; COX-2, cyclooxygenase-2; RANKL, receptor activator of nuclear factor-κB ligand.