Figure 4.

TCDD treatment increased PGE2 production and CXCR4 expression by MG-63 cells. (A) Cells were treated with toluene (vehicle control) and 0.1, 1 and 10 nM TCDD for 24 and 72 h. PGE2 protein levels in the supernatants were measured by ELISA. Data are presented as the mean ± standard error of the mean (n=3). (B) CXCR4 receptor expressions were measured by flow cytometry after 6 h treatment. M1 denoted the cells presented with CXCR4 expressions. The number represented the percentage of total cells. (C) CXCR4 expressions following treatment were measured after 6 and 24 h. *P<0.05 and **P<0.01 vs. the toluene-treated control (n=3). TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin; PGE2, prostaglandin E2.