Figure 7.

PGE2, CXCR4, CXCL12 profiles derived from MG-63 cells treated with AhR agonists. (A) Cells were treated with toluene (vehicle control), 1 or 10 nM TCDD and 10 nM TCDD plus CH-223191 for 72 h. PGE2 protein levels in the supernatants were measured by ELISA. (B) CXCR4 receptor expressions were measured by flow cytometry after toluene (vehicle control), 1 nM, 10 nM TCDD and 10 nM TCDD plus CH-223191 treatment for 24 h. The number represented the percentage of total cells. (C) CXCL12 protein production in the supernatant after cells were treated with toluene (vehicle control), 1 nM, 10 nM TCDD and 10 nM TCDD plus CH-223191 for 24 h was measured by ELISA. Data are presented as the mean ± standard error of the mean (n=3). **P<0.01 vs. the toluene-treated control (n=3). CH, 10 µM CH-223191; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin; AhR, aryl hydrocarbon receptor; PGE2, prostaglandin E2.