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. 2018 Jul 6;16(3):3569–3576. doi: 10.3892/ol.2018.9101

Figure 6.

Figure 6.

Autophagy is associated with ailanthone-mediated apoptosis of HL-60 cells. (A) Cells were pretreated with and without BaF-A1 (1 or 2 nM) for 30 min, following treatment with and without ailanthone (10 µM) for 48 h at 37°C. MTT assay was used to determine the cell viability. (B) Cells were pretreated with and without BaF-A1 (2 nM) for 30 min, following by treatment with and without ailanthone (10 µM) for 48 h at 37°C, (C) annexin V-FITC staining assay was used to analyze the cell apoptosis, ANOVA using Newman-Keuls multiple comparison test. *P<0.05 vs. control, #P<0.05 vs. (0, 10 µM) groups, **P<0.05 vs. control, ##P<0.05 vs. (0, 10 µM) groups. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide.